Send us a specification — most quotes answered within a few hours.

Organism · Solutions

Yeast and Mould testing

Yeasts and moulds are the organisms that end a shelf-life claim. They rarely make anyone ill, but they are the most common reason a product is rejected by a customer before its date has passed.

  • ISO 21527-1
  • Culture · Rapid
  • Beverage · Bakery · Dairy
Yeast and Mould colonies on selective agarFilamentous and smooth colonies · DRBC agar

Why Yeast and Mould testing is demanding

Yeasts and moulds are spoilage organisms rather than pathogens for most purposes, which changes how they are managed: the risk is commercial rather than regulatory, and it materialises after product has shipped, when a customer opens a package before its stated date.

They are also slow. Incubation typically runs five to seven days, far beyond the release window for most products, which means yeast and mould results usually validate a shelf-life claim retrospectively rather than gating a release. Getting the trend right therefore matters more than getting any single result quickly.

Low-water-activity and acidic products — dried goods, fruit preparations, juices, some dairy — are the highest-risk matrices, because those conditions suppress bacteria while leaving fungi comparatively unaffected. Selective media that suppress bacterial overgrowth are what make the count readable at all in those matrices.

Role
Spoilage indicator
Result type
Enumeration
Reference method
ISO 21527-1
Result
5–7 days

Methods compared

Slow by nature — the choice is about readability and bacterial suppression, not speed.

  1. Traditional · ISO 21527-1Selective enumeration

    Colony-count technique on dichloran rose bengal chloramphenicol agar, which suppresses bacterial growth and restricts mould spreading so colonies stay countable.

    Time to result5–7 days
  2. RapidReady-to-use plates

    Ready-poured selective formats that remove preparation and give a more consistent surface for reading filamentous colonies across a long incubation.

    Time to result5 days
  3. MolecularSpecific spoilage organisms

    PCR-based detection where a named spoilage yeast is the concern and its level is below what plate counts resolve in time to act.

    Time to resultSame day

Water activity determines the method — ISO 21527-1 above 0.95 aw, 21527-2 below.

Ask us

Products for Yeast and Mould testing

A selection spanning culture media, rapid formats and molecular assays. The full range is in the catalogue.

Easy Plate Yeast Mold-Rapid (100T/BOX)
RapidREF 22061977

Kikkoman Biochemifa

Easy plate yeast mold-rapid (100t/box)

Yeast and MouldOthers

100 tests (25 tests x 4 bags)

AOAC PTM · MicroVal

View details
Aspergillus brasiliensis ATCC 16404
TraditionalREF 0392C

Microbiologics

Aspergillus brasiliensis atcc 16404

Yeast and MouldOthers

2 vials x 10 pellets + 10 x 2 mL hydrating fluid

AOAC PTM

View details
Aspergillus brasiliensis ATCC 16404
TraditionalREF 0392L

Microbiologics

Aspergillus brasiliensis atcc 16404

Yeast and MouldOthers

6 lyophilised discs per vial

AOAC PTM · AOAC PTM

View details
Aspergillus brasiliensis derived from ATCC® 16404
TraditionalREF 0392X

Microbiologics

Aspergillus brasiliensis derived from atcc® 16404

Yeast and MouldOthers

5 units per pack

AOAC PTM

View details
Aspergillus brasiliensis derived from ATCC® 16404™ (Pack of 6)
TraditionalREF 0392K

Microbiologics

Aspergillus brasiliensis derived from atcc® 16404™ (pack of 6)

Yeast and MouldOthers

6 units per pouch

AOAC PTM · AOAC PTM

View details
Aspergillus brasiliensis derived from ATCC® 16404™ EZ-Accu Shot
TraditionalREF 0392A

Microbiologics

Aspergillus brasiliensis derived from atcc® 16404™ ez-accu shot

Yeast and MouldOthers

5 pellet vials + 5 x 1.2 mL hydrating fluid

AOAC PTM

View details
Aspergillus brasiliensis derived from ATCC® ™16404 Kwik-Stik (2Pcs)
TraditionalREF 0392P

Microbiologics

Aspergillus brasiliensis derived from atcc® ™16404 kwik-stik (2pcs)

Yeast and MouldOthers

2 units per pouch

AOAC PTM · AOAC PTM

View details
Aspergillus niger derived from ATCC® 16888™
TraditionalREF 0245K

Microbiologics

Aspergillus niger derived from atcc® 16888™

Yeast and MouldOthers

6 units per pouch

AOAC PTM · AOAC PTM

View details

Standards & compliance

The references a yeast and mould programme is measured against.

ISO 21527-1
Enumeration of yeasts and moulds in products with water activity greater than 0.95.
ISO 21527-2
Enumeration of yeasts and moulds in products with water activity less than or equal to 0.95 — dried and low-moisture goods.
SFDA
Supplied through an SFDA-licensed establishment, aligned to Kingdom requirements for the matrices you test.
ISO/IEC 17025
Suitable for accredited laboratories, with lot documentation and traceability available on request.

Sourced from the brands your methods are built on.

Japan
Italy
Italy · Germany · Hungary · US
UK

Testing for Yeast and Mould?

Tell us your matrix and how fast you need a release decision — we'll recommend the method and respond with pricing, availability and lead time.