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Pseudomonas testing

Pseudomonas is the organism that spoils chilled product and colonises water systems — psychrotrophic, biofilm-forming, and remarkably tolerant of the conditions meant to control it.

  • ISO 13720
  • Culture
  • Chilled · Water · Pharma
Fluorescent colonies · Pseudomonas agar

Why Pseudomonas testing is demanding

Pseudomonas grows at refrigeration temperature, which makes it the dominant spoilage organism in chilled protein and dairy. It produces proteases and lipases that degrade product well before any safety threshold is reached, so the practical consequence is off-flavour, slime and rejection rather than illness.

In water systems and pharmaceutical manufacturing it matters for a different reason: P. aeruginosa is an objectionable organism, and its biofilm-forming ability means it establishes itself in pipework, taps and storage vessels where routine sanitisation does not reach it.

That biofilm behaviour is what makes it persistent. Planktonic cells in a water sample may test negative while an established biofilm continues to shed intermittently, which is why Pseudomonas monitoring emphasises repeated sampling over a single clean result.

Role
Spoilage · objectionable
Result type
Enumeration
Reference method
ISO 13720
Result
24–48 h

Methods compared

Chilled spoilage and water-system control are different jobs for the same organism.

  1. Traditional · ISO 13720Selective enumeration

    Colony-count technique on selective Pseudomonas agar with CFC supplement, reading fluorescent colonies under UV.

    Time to result24–48 h
  2. RapidReady-to-use plates

    Ready-poured selective formats for the routine volume that chilled-product and water monitoring programmes generate.

    Time to result24 h
  3. Molecular · PCRP. aeruginosa confirmation

    Species-level PCR confirmation where P. aeruginosa specifically is the objectionable organism named in a pharmaceutical or water specification.

    Time to resultSame day

For water systems, sample repeatedly — biofilms shed intermittently.

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Products for Pseudomonas testing

A selection spanning culture media, rapid formats and molecular assays. The full range is in the catalogue.

ACETAMIDE Agar (500 g)
TraditionalREF 610312

Liofilchem

Acetamide agar (500 g)

Pseudomonas

500 g

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ACETAMIDE Broth (500 g)
TraditionalREF 610313

Liofilchem

Acetamide broth (500 g)

Pseudomonas

500 g

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ASPARAGINE ENRICHMENT Broth (500 g)
TraditionalREF 610138

Liofilchem

Asparagine enrichment broth (500 g)

Pseudomonas

500 gAPHA

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Acetamide Broth ISO 16266 (500 g)
TraditionalREF 610392

Liofilchem

Acetamide broth iso 16266 (500 g)

Pseudomonas

500 gEN ISO 16266:2008

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TraditionalREF 81049

Liofilchem

Cfc (pseudomonas) supplement (10 vials)

Pseudomonas

10 bottles

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TraditionalREF 81006

Liofilchem

Cn (pseudomonas) supplement (10 vials)

Pseudomonas

10 bottles

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Nutrient Agar ISO 16266 (500 g)
TraditionalREF 610036

Liofilchem

Nutrient agar iso 16266 (500 g)

Pseudomonas

500 gISO 16266:2008

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PSEUDOMONAS Agar BASE (500 g)
TraditionalREF 610071

Liofilchem

Pseudomonas agar base (500 g)

Pseudomonas

500 gISO 13720:1995

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Standards & compliance

The references a Pseudomonas programme is measured against.

ISO 13720
Enumeration of presumptive Pseudomonas spp. in meat and meat products.
ISO 16266
Detection and enumeration of Pseudomonas aeruginosa in water by membrane filtration.
SFDA
Supplied through an SFDA-licensed establishment, aligned to Kingdom requirements for the matrices you test.
ISO/IEC 17025
Suitable for accredited laboratories, with lot documentation and traceability available on request.

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Testing for Pseudomonas?

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