Description
Acetamide Broth ISO 16266 is a confirmation medium for Pseudomonas aeruginosa recovered during membrane-filtration examination of water.
Acetamide is the sole carbon source and is deaminated by non-fermenting Gram-negative bacteria to produce ammonia. The resulting alkaline reaction changes the indicator colour.
Presumptive colonies from Pseudomonas CN agar are subcultured, inoculated into the broth and incubated at 36 ± 2°C for 20–24 hours. The colour reaction is interpreted according to ISO 16266.
