Description
Acid Broth is a dehydrated culture medium for cultivating acid-tolerant microorganisms from canned foods. The medium has a final pH of 4.0 ± 0.5 and is prepared by suspending 28 g in 1 litre of water, followed by autoclaving at 121°C for 15 minutes. Samples are incubated at 55 ± 1°C for 18–48 hours, with growth indicated by turbidity.
