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Enterococcus testing

Enterococcus is a durable faecal indicator — more resistant to salt, heat and drying than E. coli, which makes it the more reliable signal in matrices where the standard indicator does not survive.

  • ISO 7899-2
  • Culture
  • Water · Food · Environmental
Red-maroon colonies · Slanetz-Bartley agar

Why Enterococcus testing is demanding

Enterococci are used as faecal indicators in the same role as E. coli, but they earn their place through durability. They tolerate salt, survive drying, and withstand heat treatment better than coliforms — so they persist where the standard indicators have already died off.

That makes them the more meaningful indicator in saline waters, processed foods, and matrices that have undergone a treatment step. A negative E. coli result in a salted or dried product may reflect the indicator failing to survive rather than an absence of contamination; enterococci close that gap.

The same durability makes them relevant to recreational and marine water quality standards, where they are frequently the primary specified indicator rather than a supplementary one. The method is straightforward enumeration; interpretation is where the judgement lies.

Role
Durable faecal indicator
Result type
Enumeration
Reference method
ISO 7899-2
Result
44–48 h

Methods compared

Chosen where standard indicators do not survive the matrix.

  1. Traditional · ISO 7899-2Membrane filtration

    Membrane filtration onto Slanetz and Bartley medium with bile aesculin azide confirmation. The reference method for water samples.

    Time to result44–48 h
  2. RapidChromogenic formats

    Chromogenic media that confirm enterococci at the reading stage, removing the separate confirmation step from routine workflows.

    Time to result24 h
  3. MolecularRarely required

    Molecular methods are uncommon here — the indicator is quantitative and culture-based enumeration is well established and economical.

    Time to resultNot typical

Consider enterococci wherever a treatment or preservation step may have killed E. coli.

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Products for Enterococcus testing

A selection spanning culture media, rapid formats and molecular assays. The full range is in the catalogue.

AZIDE DEXTROSE Broth (500 g)
TraditionalREF 610003

Liofilchem

Azide dextrose broth (500 g)

Enterococcus

500 g

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AZIDE MALTOSE Agar KF (500 g)
TraditionalREF 610154

Liofilchem

Azide maltose agar kf (500 g)

EnterococcusOthers

500 gAPHA

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BARNES Agar BASE (500 g)
TraditionalREF 610156

Liofilchem

Barnes agar base (500 g)

EnterococcusOthers

500 g

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BILE AESCULIN Agar (500 g)
TraditionalREF 610210

Liofilchem

Bile aesculin agar (500 g)

EnterococcusOthers

500 gEP 2.6.12

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BILE AESCULIN Broth (500 g)
TraditionalREF 610151

Liofilchem

Bile aesculin broth (500 g)

Enterococcus

500 g

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BILE BACTERIOLOGICAL (500 g)
TraditionalREF 611367

Liofilchem

Bile bacteriological (500 g)

EnterobacteriaceaeEnterococcus

500 g

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BROMOCRESOL PURPLE AZIDE Broth (500 g)
TraditionalREF 610321

Liofilchem

Bromocresol purple azide broth (500 g)

Enterococcus

500 g

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Bile Aesculin Azide Agar (500 g)
TraditionalREF 610001

Liofilchem

Bile aesculin azide agar (500 g)

Enterococcus

500 gISO 7899-2:2000

View details

Standards & compliance

The references an Enterococcus programme is measured against.

ISO 7899-2
Water quality — detection and enumeration of intestinal enterococci by membrane filtration.
ISO 7899-1
The miniaturised MPN counterpart for surface and waste water.
SFDA
Supplied through an SFDA-licensed establishment, aligned to Kingdom requirements for the matrices you test.
ISO/IEC 17025
Suitable for accredited laboratories, with lot documentation and traceability available on request.

Sourced from the brands your methods are built on.

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Testing for Enterococcus?

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