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Legionella testing

Legionella is a water-systems organism rather than a food one — controlled through engineering and monitored through culture, with a testing programme that answers to building safety regulation as much as to product quality.

  • ISO 11731
  • Culture · PCR
  • Water systems · Cooling towers
Grey-white colonies · GVPC agar

Why Legionella testing is demanding

Legionella colonises warm water systems — cooling towers, calorifiers, spa pools, and the stagnant legs of building water distribution. Infection is by inhalation of aerosol rather than ingestion, which is why the risk concentrates around equipment that generates spray.

Its control is fundamentally an engineering matter: temperature regimes, flow, and the elimination of dead legs. Testing verifies that control rather than delivering it, and a positive result is read as evidence that the system, not the sample, needs attention.

Culture on buffered charcoal yeast extract with selective supplements remains the reference approach, and it is slow — up to ten days — because the organism is fastidious and slow-growing. PCR provides a much faster indication, but culture remains the method regulatory frameworks reference for confirmation.

Role
Water-system safety
Result type
Enumeration
Reference method
ISO 11731
Culture result
Up to 10 days

Methods compared

Slow by nature — PCR indicates, culture confirms.

  1. Traditional · ISO 11731BCYE culture

    Membrane filtration and culture on buffered charcoal yeast extract with selective supplements. Slow, but the method regulatory frameworks reference.

    Time to resultUp to 10 days
  2. RapidSelective plating formats

    Ready-to-use GVPC formats that reduce preparation variability across a long incubation where consistency is difficult to maintain.

    Time to result7–10 days
  3. Molecular · qPCRFast system indication

    Quantitative PCR giving a same-day indication of Legionella presence — valuable for triaging a suspect system while culture confirmation runs.

    Time to resultSame day

A positive points at the system, not the sample — investigate the engineering.

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Products for Legionella testing

A selection spanning culture media, rapid formats and molecular assays. The full range is in the catalogue.

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MolecularREF 5123222601

Gold Standard Diagnostics

Bacgene legionella multiplex

Legionella

96 reactionsISO 11731:2017

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BUFFERED YEAST EXTRACT Broth (500 g)
TraditionalREF 610342

Liofilchem

Buffered yeast extract broth (500 g)

Legionella

500 gISO 11731:2017

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TraditionalREF 81056

Liofilchem

Legionella (bcye) growth suppl. (10 vials)

Legionella

10 vialsISO 11731:1998

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TraditionalREF 81091

Liofilchem

Legionella (bcye) growth suppl. w/o l-cysteine (10 vials)

Legionella

10 vialsISO 11731:1998

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TraditionalREF 81002

Liofilchem

Legionella (bmpa) supplement (10 vials)

Legionella

10 vials

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TraditionalREF 81008

Liofilchem

Legionella (gvpc) supplement (10 vials)

Legionella

10 vialsISO 11731:1998

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TraditionalREF 81019

Liofilchem

Legionella (mwy) supplement (10 vials)

Legionella

10 vialsISO 11731:2017

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TraditionalREF 81099

Liofilchem

Legionella ab supplement (10 vials)

Legionella

10 vialsISO 11731:2017

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Standards & compliance

The references a Legionella programme is measured against.

ISO 11731
Water quality — enumeration of Legionella. The reference culture method for water system samples.
ISO/TS 12869
Detection and quantification of Legionella spp. by concentration and genic amplification (qPCR).
SFDA
Supplied through an SFDA-licensed establishment, aligned to Kingdom requirements for the matrices you test.
ISO/IEC 17025
Suitable for accredited laboratories, with lot documentation and traceability available on request.

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Testing for Legionella?

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