Description
SIM Agar ISO 15213-2 is a semisolid confirmation medium for Clostridium perfringens in food, feed, environmental and primary-production samples.
Ferrous ammonium sulfate and sodium thiosulfate demonstrate sulfite reduction through black precipitate formation. The low agar concentration permits observation of motility, and Kovac's reagent is used to assess indole production.
A pure colony is stab-inoculated and incubated anaerobically at 37 ± 1°C for 20–24 hours. Clostridium perfringens is characterised by blackening without motility and without an indole-positive red ring.
