Description
EMB Agar with Lactose and Sucrose is a selective differential medium for isolating enteric Gram-negative bacteria.
Eosin inhibits many Gram-positive bacteria and works with methylene blue to indicate lactose or sucrose fermentation. Lactose fermenters form blue-black colonies, while Escherichia coli may show a green metallic sheen.
Clinical specimens are streaked for isolation and incubated at 35 ± 2°C for 18–24 hours. Additional biochemical tests are required for definitive identification.
