Description
Slanetz Bartley Agar with TTC is a selective membrane-filtration and direct-plating medium for detecting and enumerating intestinal enterococci.
Sodium azide suppresses competing microorganisms and TTC is reduced by enterococci to red formazan, producing red to maroon colonies.
A sample membrane or dilution is placed on the complete medium and incubated at 36 ± 2°C for approximately 40–48 hours. Characteristic colonies are counted and may be confirmed on bile-aesculin-azide agar.
