Description
Tergitol Agar Base is a selective differential membrane-filtration medium for detection and enumeration of Escherichia coli and coliform bacteria in water.
Tergitol inhibits Gram-positive organisms, lactose fermentation changes bromothymol blue, and TTC reduction produces red formazan reactions that aid colony differentiation.
A water sample is membrane-filtered, the membrane is placed on completed agar and plates are incubated at 35 ± 2°C for 18–24 hours. Characteristic colonies are counted and confirmed.
