Description
Nitrate Broth is a biochemical medium for determining nitrate-reduction activity in cultured bacteria.
Organisms capable of reducing nitrate form nitrite or further-reduced products. After incubation, the specified sulfanilic-acid and alpha-naphthylamine reagents produce a red-to-orange reaction when nitrite is present.
A pure culture is inoculated into the broth and incubated before the nitrate reagents are added. The result is interpreted with the additional confirmation step described in the PDF.
