Description
Reinforced Clostridial Medium is a non-selective semi-solid medium for cultivating and enumerating clostridia and other anaerobic bacteria.
Peptones and extracts supply nutrients, cysteine creates reducing conditions, starch detoxifies metabolites and the low agar concentration supports a semi-solid anaerobic environment.
Pure cultures are stab-inoculated and incubated anaerobically at 35 ± 2°C for 18–48 hours. Turbidity indicates growth.
