Description
Middlebrook 7H10 Agar Base is a moderately selective solid medium for isolating and cultivating Mycobacterium species, particularly Mycobacterium tuberculosis.
Malachite green limits contaminating flora. Glycerol and OADC supplement provide energy, protective albumin, oleic acid, catalase and other growth factors required by mycobacteria.
Processed specimens are inoculated and incubated at 35 ± 2°C in a carbon-dioxide-enriched atmosphere for up to eight weeks, with cultures examined periodically.
