Description
Iron Sulphite Agar is a differential medium for detecting and enumerating sulfite-reducing bacteria, including clostridia.
Sodium sulfite is reduced to sulfide by positive organisms, and the sulfide reacts with iron to form black colonies or blackening in the surrounding medium.
Samples and dilutions are inoculated according to ISO 15213-1 and incubated under anaerobic conditions. Characteristic black colonies are counted and confirmed where required.
